Western blot analysis of extracts from NIH/3T3 cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Acetyl-CoA Carboxylase 1 siRNA I #6397 (Mouse Specific) (+), or SignalSilence ® Acetyl-CoA Carboxylase 1 siRNA II (Mouse Specific) (+), using Acetyl-CoA Carboxylase 1 Antibody #4190 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Acetyl-CoA Carboxylase 1 Antibody confirms silencing of Acetyl-CoA Carboxylase 1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.